Spectrophotometric Simultaneous Estimation of Salbutamol and Prednisolone in Bulk and Formulation

Combination of Salbutamol and Prednisolone was found effective for the treatment of acute and chronic asthma. A fast, simple and accurate method for the estimation of this combination is necessary so, two UV Spectrophotometric methods for simultaneous estimation of salbutamol (SAL) and prednisolone (PRE) have been developed in the present work. First method is Q value analysis based on measurement of absorptivity at 230nm (iso-absorptive point) and 244nm (λ_max for Prednisolone) as two analytical wavelengths for both drugs in methanol. Second method involves multicomponent mode of analysis, wavelength selected are 227nm (λ_max for Salbutamol) and 244 nm (λ_max for Prednisolone). All the methods show linearity in the concentration range of 6-20μg/ml for salbutamol as well as for prednisolone. The accuracy and precision of the methods were determined and validated stastically which shows no significant difference between the results obtained by the two methods. The proposed methods are rapid, simple and accurate and can be used for its intended purpose.