Mechanistic Approach to Determination of Anti-diabetic Activity of Calystegia Sepium R.Br. Flowering Plants in Normal and Streptozotocin Induced Rats

The Hypoglycemic effect of water and ethanol extracts of Calystegia sepium flowering plants has been evaluated and compared its Hypoglycemic effect of flowering plants with Glibenclamide in Euglycemic and Hyperglycemic Wistar rats. Male Wistar rats were orally administered Alcoholic and Water extracts of Calystegia sepium flowering plant and investigated for Hypoglycemic activity in Normal and Streptozotocin (STZ)-induced diabetic rats and compared their effect with Glibenclamide [Single dose one day study and multiple dose15 day study] Blood glucose levels were estimated at 0^th, 5^th, 10^th and 15^th day by Kit method and on 15^th day lipid profiles were also estimated and other parameters like insulin estimation, insulin tolerance test, and glucose uptake by isolated hemi-diaphragm was estimated. The alcoholic and water extract of Calystegia sepium flower in plant exhibited significant Hypoglycemic and Hypolipidemic effect in Euglycemic and Hyperglycemic rats. The study reveals the hypoglycemic and hypolipidemic activity of alcoholic and water extract of Calystegia sepium flowering plants in euglycemic and Streptozotocin induced diabetic rats. The extract seems promising for the development of a phyto-medicine for a Diabetes mellitus.