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Evaluation of Peptide-Based Approach for Estimation of Nse and S-100ββ towards the Development of a Cost-Effective Test for Prognosis of AIS Patients


Affiliations
1 Biochemistry Research Centre, Central India Institute of Medical Sciences, Nagpur 440 020, India
2 Environmental Genomics Unit, National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440 020, India
 

Here we compare the in-house ELISA technique for estimation of neuron-specific enolase (NSE) and S-100ββ (glial-specific protein) peptides with a commercial kit in serum samples of acute ischaemic stroke (AIS) patients. Nineteen improved and four expired AIS patients were included for the study. NSE concentrations were significantly higher (P < 0.05) in expired as compared with improved AIS patients by both inhouse ELISA and commercial kit method. Estimation of S-100ββ by only in-house ELISA showed significantly high (P < 0.05) levels in expired AIS patients. Peptide-based estimation of NSE and S-100ββ may be used for prognosis of AIS patients.

Keywords

Acute Ischaemic Stroke, Glial-Specific Protein, Neurological Disease, Neuron-specific Enolase, Prognosis.
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  • Evaluation of Peptide-Based Approach for Estimation of Nse and S-100ββ towards the Development of a Cost-Effective Test for Prognosis of AIS Patients

Abstract Views: 314  |  PDF Views: 141

Authors

Amit R. Nayak
Biochemistry Research Centre, Central India Institute of Medical Sciences, Nagpur 440 020, India
Neha H. Lande
Biochemistry Research Centre, Central India Institute of Medical Sciences, Nagpur 440 020, India
Anuja P. Kawle
Biochemistry Research Centre, Central India Institute of Medical Sciences, Nagpur 440 020, India
Dinesh P. Kabra
Biochemistry Research Centre, Central India Institute of Medical Sciences, Nagpur 440 020, India
Nitin H. Chandak
Biochemistry Research Centre, Central India Institute of Medical Sciences, Nagpur 440 020, India
Hemant J. Purohit
Environmental Genomics Unit, National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440 020, India
Girdhar M. Taori
Biochemistry Research Centre, Central India Institute of Medical Sciences, Nagpur 440 020, India
Hatim F. Daginawala
Biochemistry Research Centre, Central India Institute of Medical Sciences, Nagpur 440 020, India
Rajpal S. Kashyap
Biochemistry Research Centre, Central India Institute of Medical Sciences, Nagpur 440 020, India

Abstract


Here we compare the in-house ELISA technique for estimation of neuron-specific enolase (NSE) and S-100ββ (glial-specific protein) peptides with a commercial kit in serum samples of acute ischaemic stroke (AIS) patients. Nineteen improved and four expired AIS patients were included for the study. NSE concentrations were significantly higher (P < 0.05) in expired as compared with improved AIS patients by both inhouse ELISA and commercial kit method. Estimation of S-100ββ by only in-house ELISA showed significantly high (P < 0.05) levels in expired AIS patients. Peptide-based estimation of NSE and S-100ββ may be used for prognosis of AIS patients.

Keywords


Acute Ischaemic Stroke, Glial-Specific Protein, Neurological Disease, Neuron-specific Enolase, Prognosis.

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DOI: https://doi.org/10.18520/cs%2Fv109%2Fi8%2F1466-1470