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Studies on Regulation of Xylanase Bio-Synthesis by Trichoderma reesei SAF3


Affiliations
1 Department of Botany, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, India
2 PG Department of Botany, Ramananda College, Bishnupur, Bankura, 722122, West Bengal, India
3 Department of Physiology, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, India
4 Department of Microbiology, Vidyasagar University, Paschim Medinipur 721102, West Bengal, India
     

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Microbial xylanases have an exciting potentiality and are being used extensively in different industries. In this study, regulation of xylanase bio-synthesis was examined in Trichoderma reesei SAF3. Xylan (1.0% w/v) showed the highest inductive effect. Glucose at concentration of 1% (w/v) was repressive for xylanase bio-synthesis when used in presence or absences of xylan; the repression was partially elevated by addition of cyclic adenosine monophosphate (cAMP, 5mM). The above findings suggested that xylan was the major inducer and the induction was energy dependent, mediated by second messenger system.

Keywords

Xylanase, Regulation of Biosynthesis, cAMP, Trichoderma Reesei.
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  • Studies on Regulation of Xylanase Bio-Synthesis by Trichoderma reesei SAF3

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Authors

Sanjay Kar
Department of Botany, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, India
Asish Mandal
PG Department of Botany, Ramananda College, Bishnupur, Bankura, 722122, West Bengal, India
Saptadip Samanta
Department of Physiology, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, India
Bikash Ranjan Pati
Department of Microbiology, Vidyasagar University, Paschim Medinipur 721102, West Bengal, India
Keshab Chandra Mondal
Department of Microbiology, Vidyasagar University, Paschim Medinipur 721102, West Bengal, India

Abstract


Microbial xylanases have an exciting potentiality and are being used extensively in different industries. In this study, regulation of xylanase bio-synthesis was examined in Trichoderma reesei SAF3. Xylan (1.0% w/v) showed the highest inductive effect. Glucose at concentration of 1% (w/v) was repressive for xylanase bio-synthesis when used in presence or absences of xylan; the repression was partially elevated by addition of cyclic adenosine monophosphate (cAMP, 5mM). The above findings suggested that xylan was the major inducer and the induction was energy dependent, mediated by second messenger system.

Keywords


Xylanase, Regulation of Biosynthesis, cAMP, Trichoderma Reesei.

References





DOI: https://doi.org/10.24906/isc%2F2019%2Fv33%2Fi3%2F185424