International Journal of Plant Sciences

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Studies on Anther Culture in Tomato


Affiliations
1 Department of Genetics and Plant Breeding, Vasantrao Naik Marathwada Agricultural University, Parbhani (M.S.), India
2 Department of Genetics and Plant Breeding, Agriculture Technical School, Buldana (M.S.), India
     

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Anther or pollen culture have been used in mutation and F1 hybrid breeding programme in many plant species. In order to get haploid plants, three tomato varieties were used in this study. Anther were removed from 2-4 mm, 5-6 mm and 8-10 mm length tomato flowers. Two different nutrient media were investigated to get callus. N6 medium+2 mg/L NAA+1 mg/L Kinetin was most efficient medium for anther callus growth. Calluses were subcultured but calli did not show any response for further callus growth and haploid plantlets were not obtained.

Keywords

Anther Culture, Callus Formation, Tomato.
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  • Studies on Anther Culture in Tomato

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Authors

U. B. Shere
Department of Genetics and Plant Breeding, Vasantrao Naik Marathwada Agricultural University, Parbhani (M.S.), India
N. R. Mairan
Department of Genetics and Plant Breeding, Agriculture Technical School, Buldana (M.S.), India

Abstract


Anther or pollen culture have been used in mutation and F1 hybrid breeding programme in many plant species. In order to get haploid plants, three tomato varieties were used in this study. Anther were removed from 2-4 mm, 5-6 mm and 8-10 mm length tomato flowers. Two different nutrient media were investigated to get callus. N6 medium+2 mg/L NAA+1 mg/L Kinetin was most efficient medium for anther callus growth. Calluses were subcultured but calli did not show any response for further callus growth and haploid plantlets were not obtained.

Keywords


Anther Culture, Callus Formation, Tomato.

References