Callus formation in Ferula jaeschkeana Vatke

Callus was initiated from the petiole explants of Ferula jaeschakeana on a medium containing 2,4-D. The highest percentage of explants forming callus (92.5) and callus index (258) were obtained on medium supplemented with 1 mgl^-1 2,4-D. Among explants sizes and type tested for callus initiation, 10 mm long petiole gave maximum callus index. Small and large explants initiated a slight amount of callus. Addition of Kn in combination with 2,4-D enhanced the callus formation from the explants.