Journal of Surface Science and Technology

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Concentration of Capping Agent Controls Size Selection, Agglomeration and Antimicrobial Action of Silver Nanoparticles


Affiliations
1 Department of Microbiology, Institute of Post Graduate Medical Education and Research, 244 Acharya Jagadish Chandra Bose Road, Kolkata – 700020, West Bengal, India
2 Advanced Mechanical and Materials Characterization Division, CSIR-Central Glass and Ceramic Research Institute, 196, Raja Subodh Chandra Mullick Road, Kolkata – 700032, West Bengal, India
     

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Polyvinyl Alcohol (PVA) capped Ag nanoparticles (AgNPs) formed through reduction of Silver Nitrate (AgNO3) by Sodium Borohydride (NaBH4) were studied for different PVA concentrations (C, %w/w of solution) in the bulk. Diameter (DAg) of Ag metallic core, obtained from plasmon resonance in Ultraviolet-Visible spectra, had a peak (≈12.8 nm) at C~0.24, which almost exactly matched the gyration sphere diameter of PVA molecule (∼13 nm). Cluster diameter (Dcl), measured after a month through Dynamic Light Scattering (DLS) study, gave the number of NPs per cluster showing a dip at C∼0.24. Minimum inhibitory concentration (MIC) of AgNPs on Escherichia coli (E. coli), assayed from standard broth dilution method (CLSI M07-A8), showed MIC of 0.66 μg/ml at the PVA concentration of 0.24%. Scanning Electron Microscopy (SEM) showed NP clusters accumulating over E. coli to have Dcl ∼ DAg whereas those lying outside the bacteria to have Dcl ∼ 3DAg, consistent with a strong size selectivity of antimicrobial efficacy.

Keywords

Coalescence, Minimum Inhibitory Concentration, Polyvinyl Alcohol, Silver Nanoparticles.
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  • Concentration of Capping Agent Controls Size Selection, Agglomeration and Antimicrobial Action of Silver Nanoparticles

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Authors

Rehana Parveen
Department of Microbiology, Institute of Post Graduate Medical Education and Research, 244 Acharya Jagadish Chandra Bose Road, Kolkata – 700020, West Bengal, India
Alokmay Datta
Advanced Mechanical and Materials Characterization Division, CSIR-Central Glass and Ceramic Research Institute, 196, Raja Subodh Chandra Mullick Road, Kolkata – 700032, West Bengal, India
Prasanta Kumar Maiti
Department of Microbiology, Institute of Post Graduate Medical Education and Research, 244 Acharya Jagadish Chandra Bose Road, Kolkata – 700020, West Bengal, India

Abstract


Polyvinyl Alcohol (PVA) capped Ag nanoparticles (AgNPs) formed through reduction of Silver Nitrate (AgNO3) by Sodium Borohydride (NaBH4) were studied for different PVA concentrations (C, %w/w of solution) in the bulk. Diameter (DAg) of Ag metallic core, obtained from plasmon resonance in Ultraviolet-Visible spectra, had a peak (≈12.8 nm) at C~0.24, which almost exactly matched the gyration sphere diameter of PVA molecule (∼13 nm). Cluster diameter (Dcl), measured after a month through Dynamic Light Scattering (DLS) study, gave the number of NPs per cluster showing a dip at C∼0.24. Minimum inhibitory concentration (MIC) of AgNPs on Escherichia coli (E. coli), assayed from standard broth dilution method (CLSI M07-A8), showed MIC of 0.66 μg/ml at the PVA concentration of 0.24%. Scanning Electron Microscopy (SEM) showed NP clusters accumulating over E. coli to have Dcl ∼ DAg whereas those lying outside the bacteria to have Dcl ∼ 3DAg, consistent with a strong size selectivity of antimicrobial efficacy.

Keywords


Coalescence, Minimum Inhibitory Concentration, Polyvinyl Alcohol, Silver Nanoparticles.

References





DOI: https://doi.org/10.18311/jsst%2F2020%2F24875