Research Journal of Pharmacology and Pharmacodynamics

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Effect of Acacia catechu and Rotula aquatica on the DNA: Implications for Cancer Therapy


Affiliations
1 Department of Pharmacognosy and Phytochemistry, Principal K M Kundnani College of Pharmacy, Cuffe Parade, Mumbai-400 005, India
2 Principal, Singhgad's Institute of Pharmaceutical Sciences, Kusgaon (BK), Lonavala, India
3 Department of Genetics, BARC, Mumbai, India
4 Orchid Research Laboratories Ltd. 476, Chennai 600119, India
     

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Ethnobotanical search has revealed the use of water extracts of bark of Acacia catechu and Rotula aquatica in the treatment of cancer. The aqueous extracts of both above mentioned plants were evaluated for antimitotic activity using the meristamatic cells of Allium cepa ischolar_mains. The results showed inhibition of prophase stages in cell division. The A.catechu and R. aquatica treated ischolar_mains of A.cepa were treated with tritiated thymidine. DNA was extracted from these ischolar_main tips. The total DNA was then counted in a scintillation counter. The results were compared with a positive control Methotrexate, a known anticancer drug. Roots treated with Plain water were used as negative control. The total DNA count from the treated ischolar_mains and that from Methotrexate treated ischolar_mains was less than that of Plain water. The extracted DNA was subjected to gel electrophoresis. DNA from ischolar_mains treated with extracts and from Methotrexate treated ischolar_mains showed fragmentation where as that from the Plain water was intact.

Keywords

Acacia catechu, Rotula aquatica, Allium cepa, Antimitotic Activity, DNA Isolation.
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  • Effect of Acacia catechu and Rotula aquatica on the DNA: Implications for Cancer Therapy

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Authors

Swati Patil
Department of Pharmacognosy and Phytochemistry, Principal K M Kundnani College of Pharmacy, Cuffe Parade, Mumbai-400 005, India
S. R. Naik
Principal, Singhgad's Institute of Pharmaceutical Sciences, Kusgaon (BK), Lonavala, India
V. Joshi
Department of Genetics, BARC, Mumbai, India
C. I . Jolly
Department of Pharmacognosy and Phytochemistry, Principal K M Kundnani College of Pharmacy, Cuffe Parade, Mumbai-400 005, India
S. Narayanan
Orchid Research Laboratories Ltd. 476, Chennai 600119, India

Abstract


Ethnobotanical search has revealed the use of water extracts of bark of Acacia catechu and Rotula aquatica in the treatment of cancer. The aqueous extracts of both above mentioned plants were evaluated for antimitotic activity using the meristamatic cells of Allium cepa ischolar_mains. The results showed inhibition of prophase stages in cell division. The A.catechu and R. aquatica treated ischolar_mains of A.cepa were treated with tritiated thymidine. DNA was extracted from these ischolar_main tips. The total DNA was then counted in a scintillation counter. The results were compared with a positive control Methotrexate, a known anticancer drug. Roots treated with Plain water were used as negative control. The total DNA count from the treated ischolar_mains and that from Methotrexate treated ischolar_mains was less than that of Plain water. The extracted DNA was subjected to gel electrophoresis. DNA from ischolar_mains treated with extracts and from Methotrexate treated ischolar_mains showed fragmentation where as that from the Plain water was intact.

Keywords


Acacia catechu, Rotula aquatica, Allium cepa, Antimitotic Activity, DNA Isolation.

References