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Anti-inflammatory Activities of Methanolic Extracts from Melastoma Malabathricum Linn. and Melastoma Decemfidum Roxb. Leaves on Macrophage RAW264.7 Cell Line
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Two plant species from the Melastomataceae family, Melastomamalabathricum Linn. and Melastomadecemfidum Roxb, which grow in abundance in tropical and subtropical regions have been well-documented to possess various medicinal values including anti-inflammatory properties. The present study was undertaken to examine the anti-inflammatory potential of M. Malabathricum and M. Decemfidum methanolic extracts (MME and MDE respectively) on lipopolysaccharide (LPS)-induced macrophage cell line (RAW 264.7). The cytotoxic assays of MME and MDE against RAW264.7 cells were conducted using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS). Production of nitric oxide (NO) from both Melastoma sp. was measured using Griess assay. MME and MDE exhibited high cell viability (>90%) at concentrations from 0.39 to 1.56μg/mL and 6.25 μg/mL respectively. Although the results obtained were not significantly different from control,NO production by LPS-induced RAW264.7 cells were observed to be lower for both extracts at concentration of1.5μg/mL when compared to untreated cells (control). The present study confirmed that MME and MDE have the potential to inhibit anti-inflammatory mediator against LSP-induced RAW264.7 cells.
Keywords
Anti-inflammatory, Melastomamalabathricum, Melastomadecemfidum, RAW264.7 Cell line.
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