Indian Journal of Public Health Research & Development

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Sperm Preparation by using Microfluidic Sperm Sorting Device Increase ICSI Success Rate


Affiliations
1 Research Scholar, Department of Anatomy, Sree Balaji Dental College & Hospital, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India
2 Govindarajan Sumathy, Prof & HOD, Department of Anatomy, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education, Chennai, India
     

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Highly motile sperms with good morphology are more capable of fertilizing an oocyte and increase the pregnancy rate during the process of ART. In swim up method and density gradient method, multiple centrifugation leads to ROS production that can cause DNA damage to the sperms. Microfluidic chips are one of the Method of sperm separation to eliminate DNA fragmentation in sperm. It is thought that the separation of sperm by centrifugation method used in sperm separation in IVF (in vitro fertilization) laboratories leads to sperm DNA fragmentation. By using microfluidics sperm sorting device, spermatozoa do not undergo such physical stress. Therefore it is possible to obtain sperms with ICSI criteria of good motility and in higher concentration with the use of micro fluidic sperm sorting device.

Keywords

Sperm Concentration, Motility, Oligozoospermia, Asthenospermia, Micro-Fluidic Sperm Sorting Device and ICSI.
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  • Sperm Preparation by using Microfluidic Sperm Sorting Device Increase ICSI Success Rate

Abstract Views: 274  |  PDF Views: 0

Authors

Ganesh Sivaraja
Research Scholar, Department of Anatomy, Sree Balaji Dental College & Hospital, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India
Sumathy Govindarajan
Govindarajan Sumathy, Prof & HOD, Department of Anatomy, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education, Chennai, India

Abstract


Highly motile sperms with good morphology are more capable of fertilizing an oocyte and increase the pregnancy rate during the process of ART. In swim up method and density gradient method, multiple centrifugation leads to ROS production that can cause DNA damage to the sperms. Microfluidic chips are one of the Method of sperm separation to eliminate DNA fragmentation in sperm. It is thought that the separation of sperm by centrifugation method used in sperm separation in IVF (in vitro fertilization) laboratories leads to sperm DNA fragmentation. By using microfluidics sperm sorting device, spermatozoa do not undergo such physical stress. Therefore it is possible to obtain sperms with ICSI criteria of good motility and in higher concentration with the use of micro fluidic sperm sorting device.

Keywords


Sperm Concentration, Motility, Oligozoospermia, Asthenospermia, Micro-Fluidic Sperm Sorting Device and ICSI.