Inhibitory Effects of Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP) and Ciprofloxacin on the Gene Expression of Nor a Efflux Pump and Reduce Antibiotic Resistance in Staphylococcus aureus

One of the important pathogen with increasing resistance rate in hospitalized patients is Staphylococcus aureus. The useful results for antimicrobial activity of CCCP made it a proper candidate to enhance the inhibitory effect of some certain antibiotics like Ciprofloxacin. The aim of this study was to investigate the Inhibitory effects of CCCP on the gene expression of norA efflux pump and decrease Ciprofloxacin resistance in Staphylococcus aureus. Thirtyisolates ofS. aureus were acquired from different clinical specimens at the Baquba teaching hospital. Susceptibility test to ciprofloxacin was done by disk diffusion test and broth dilution method. Activity of the efflux pump was recognized using CCCP as an chemical efflux pump inhibotor. MIC of CCCP was evaluated with muller hinton Broth dilution method. Bacterial culture was performed and nor A gene expression was examined by Taqman qRT-PCR by one-step and .The expression of norA was significantly decreased in these isolates when were treated with CCCP & Cip. Our results showed that CCCPcan increase ciprofloxacin susceptibility through inhibition of the norA efflux pump. Combination of CCCP with ciprofloxacin can reduce the antibiotic resistance.