Biocompatible Formulation of Probiotic Bacteria Bacillus subtilis with Agriculture Based Products for the Effective Acid Tolerance Properties and Synergistic Activity with Antibiotics

The present study was aimed to evaluate the acid tolerance properties and compatibility with antibacterial antibiotics of probiotic bacterial strain Bacillus subtilis formulated with various agriculture based products like potato peel and biogel. Bacterial strain was isolated from butter milk and the isolated probiotic strain was identified based on morphological, cultural and molecular characteristics which reveals the isolated strain belong to B.subtilis and the pure culture was formulated with the respective agriculture based products under aseptic condition. Acid tolerance study was carried out by determination of growth of respective formulation under different pH levels (6.5,4.5,2.5) which shows all the tested formulation retained the maximum viability in all the tested pH under all the incubation time. Compatibility of respective formulation with antibiotics was also investigated by measuring the zone of inhibition. Growth inhibition was not recorded in the majority of the antibiotics which reveals the best compatibility of formulation with the antibiotics which would suggests the possible utilization of formulated B.subtilis as an effective probiotic strain.